胡麻油烟凝聚物诱导人胚肺二倍体细胞恶性转化作用

Malignant Transformation of the Human Embryo Lung Diploid Cell Induced by Siritch Oil Fume Aggregates

背景与目的：研究胡麻油烟对人体潜在的致癌危险性。材料与方法：以胡麻油烟凝聚物（SOFA）作为受试物染毒人胚肺二倍体细胞，建立体外恶性转化实验系统，对转化细胞进行ConA凝集实验、软琼脂培养实验以及裸鼠体内致瘤实验，观察并检测细胞是否发生恶性变。结果：实验剂量范围内的SOFA（25～400μg／ml）能够诱导人胚肺二倍体细胞形成细胞恶性转化灶，转化灶细胞生长失去接触抑制性、饱和密度增大、ConA凝集能力增强、锚着依赖性丧失及裸鼠体内具有成瘤性等多种细胞发生恶性变的生物学特性改变。结论：SOFA具有诱导人胚肺二倍体细胞恶性转化的作用，对人体具有潜在致癌作用。

BACKGROUND ＆ AIM： To study the potential carcinogenic risk of siritch oil fume condensate （SOFC） to human. MATERIALS AND METHODS： Different concentrations of SOFC were co-incubated with human embryo lung diploid cells. The degree of malignant transformation was observed by assessing the biological characteristics of the cells. RESULTS： The concentration of SOFC within the dose range of the experiment（25- 400 μg/ml） could induce malignant transformation of the human embryo lung diploid cell, and with an obvious dose-response relationship. Transformed cells exhibited many characteristics associated with malignant transformation, such as losing contact-dependent inhibition, saturated density angmentation, growing at low serum concentration, agglutinating by different concentrations of ConA, losing anchorage dependence, forming tumor at inoculation site of athymic mouse. CONCLUSION： SOFC could induce human embryo lung diploid cell malignant transformation and showed potential carcinogenicity to human.