硒对丝裂霉素C致淋巴细胞遗传损伤的影响

Effects of Selenium on Genetic Damage Induced by Mitomycin-C in Human Peripheral Lymphocytes

背景与目的:观察硒对丝裂霉素C(mitomycin-C,MMC)诱导的人外周血淋巴细胞遗传损伤是否具有保护作用。材料与方法:在体外培养的淋巴细胞中,24h时分别加入不同剂量的硒0、1、6和10μmol/L,MMC0和0.1mg/L,培养72h后,分别采用染色体畸变分析(CA)、姐妹染色单体互换技术(SCE),以及彗星试验(SCGE),对硒能否抑制MMC诱导的外周血淋巴细胞遗传损伤进行检测。结果:(1μmol/L和6μmol/L硒+MMC)组的淋巴细胞平均尾长、SCE互换率和CA畸变率低于阳性对照组,差异有统计学意义(P<0.05)。(6μmol/L硒+MMC)组的DNA损伤程度低于(1μmol/L硒+MMC)组和(10μmol/L硒+MMC)组。结论:硒对MMC造成的人外周血淋巴细胞遗传损伤具有一定的保护作用,在本实验条件下,以6μmol/L硒组的保护作用最强。

BACKGROUND ＆ AIM： To assess the effects of selenium on MMC-induced DNA damage in human peripheral lymphoeytes. MATERIALS AND METHODS： The effects of selenium on the DNA damage and chromosome damage induced by MMC were evaluated using single cell gel electrophoresis（SCGE）, sister chromatid exchange（SCE） and chromosome aberrations （CA） in human peripheral lymphoeytes. RESULTS： The tail length , SCE frequencies and the chromosomal aberration rate of three doses of selenium were significantly lower than that of the positive control. Furthermore, the extent of DNA damage of medium dose group of selenium（6 μmol/ L） was lower than the other groups, CONCLUSION： To some extent, selenium could protect DNA damage induced by MMC. The protective effect of medium dose of selenium （6 μmol/L） was stronger than the other doses.