摘要
背景与目的:研究阿魏蘑菇(pleurotassapidus,PS)提取物对5-氟尿嘧啶(5-Fu)化疗效果的影响,并分析其作用机制。材料与方法:建立移植性人宫颈癌BALB/c(nu/nu)裸鼠模型,并随机分为模型对照组、5-Fu组、PS组、5-Fu+PS组4组,分别给予不同的药物干预,14d后结束实验,检测各项指标。结果:模型组裸鼠体内瘤体积、瘤重明显高于各干预组(P<0.01),5-Fu+PS处理组荷瘤裸鼠体内瘤重和瘤体积分别为(0.19±0.03)g,(0.134±0.016)cm3低于5-Fu处理组[(0.30±0.03)g,(0.210±0.018)cm3];5-Fu组外周血白细胞数(3.52±0.43)×109/L明显低于5-Fu+PS组(5.00±0.74)×109/L和模型组(5.51±0.50)×109/L;5-Fu+PS组瘤组织Bax基因和caspase-3蛋白表达水平强于5-Fu组(P<0.05)。结论:阿魏蘑菇具有增强5-Fu对肿瘤生长的抑制作用,和减轻5-Fu抑制白细胞增殖的功效,其机制可能与其激活Bax/caspase3途径有关。
BACKGROUND & AIM: To study the effects and mechanisms of pleurotas sapidus(PS) extract on function of 5-fluorouracil(5-Fu) in chemotherapy. MATERIALS AND METHODS: Nude mice transplanted with human cervical carcinoma were divided into four groups randomly,model group , 5-Fu group , PS group and PS+5-Fu group. After 14 days the markers in nude mice were assessed. RESULTS: The weight and volume of tumor in model group were higher than that in other three groups. The weight and volume of tumor in PS+5-Fu group(0.19±0.03) g and (0.134±0.016) cm^3, respectively, were lower than that in 5-Fu group(0.30±0.03) g and (0.210±0.018) cm^3, respectively.The number of leukocytes in peripheral blood in 5-Fu group(3.52±0.43) × 10^9/L was lower than that in PS+5-Fu group(5.00±0.74) ×10^9/L and model group(5.51±0.50) × 10^9/L. Significantly higher expressions of Bax and caspase-3 protein of tumour tissues were observed in nude mice exposed to combination of 5-Fu plus PS compared with the nude mice exposed to 5-Fu alone. CONCLUSION: The extract from pleurotas sapidus had the synergistic antitumor effect and increased the number of leukocytes in 5-Fu chemotherapy, which were related to the upregulation of Bax and caspase-3 protein expressions.
出处
《癌变.畸变.突变》
CAS
CSCD
2007年第1期59-63,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
关键词
阿魏蘑菇
5-氟尿嘧啶
化疗
pleurotas sapidus
5-fluorouracil
chemotherapy