阿魏蘑菇提取物对5-氟尿嘧啶疗效的影响

Effect of Pleurotas Sapidus Extract on 5-Fu Chemotherapeutic Function

背景与目的:研究阿魏蘑菇(pleurotassapidus,PS)提取物对5-氟尿嘧啶(5-Fu)化疗效果的影响,并分析其作用机制。材料与方法:建立移植性人宫颈癌BALB/c(nu/nu)裸鼠模型,并随机分为模型对照组、5-Fu组、PS组、5-Fu+PS组4组,分别给予不同的药物干预,14d后结束实验,检测各项指标。结果:模型组裸鼠体内瘤体积、瘤重明显高于各干预组(P<0.01),5-Fu+PS处理组荷瘤裸鼠体内瘤重和瘤体积分别为(0.19±0.03)g,(0.134±0.016)cm3低于5-Fu处理组[(0.30±0.03)g,(0.210±0.018)cm3];5-Fu组外周血白细胞数(3.52±0.43)×109/L明显低于5-Fu+PS组(5.00±0.74)×109/L和模型组(5.51±0.50)×109/L;5-Fu+PS组瘤组织Bax基因和caspase-3蛋白表达水平强于5-Fu组(P<0.05)。结论:阿魏蘑菇具有增强5-Fu对肿瘤生长的抑制作用,和减轻5-Fu抑制白细胞增殖的功效,其机制可能与其激活Bax/caspase3途径有关。

BACKGROUND ＆ AIM： To study the effects and mechanisms of pleurotas sapidus（PS） extract on function of 5-fluorouracil（5-Fu） in chemotherapy. MATERIALS AND METHODS： Nude mice transplanted with human cervical carcinoma were divided into four groups randomly,model group , 5-Fu group , PS group and PS＋5-Fu group. After 14 days the markers in nude mice were assessed. RESULTS： The weight and volume of tumor in model group were higher than that in other three groups. The weight and volume of tumor in PS＋5-Fu group（0.19±0.03） g and （0.134±0.016） cm^3, respectively, were lower than that in 5-Fu group（0.30±0.03） g and （0.210±0.018） cm^3, respectively.The number of leukocytes in peripheral blood in 5-Fu group（3.52±0.43） × 10^9/L was lower than that in PS＋5-Fu group（5.00±0.74） ×10^9/L and model group（5.51±0.50） × 10^9/L. Significantly higher expressions of Bax and caspase-3 protein of tumour tissues were observed in nude mice exposed to combination of 5-Fu plus PS compared with the nude mice exposed to 5-Fu alone. CONCLUSION： The extract from pleurotas sapidus had the synergistic antitumor effect and increased the number of leukocytes in 5-Fu chemotherapy, which were related to the upregulation of Bax and caspase-3 protein expressions.