壳聚糖对大鼠的致畸胎试验

Teratogenicity of Chitosan in SD Rats

背景与目的：通过在胚胎器官形成期连续给药，观察壳聚糖对大鼠的胚胎毒性和致畸毒性。材料与方法：将90只妊娠SD大鼠按时间先后随机分为5组，即阴性对照组、壳聚糖50、250、1000mg／kg剂量组和阳性对照组，每组18只。在大鼠胚胎器官形成期（妊娠的第6-15d），壳聚糖各剂量组和阴性对照组连续给受试物10d，每日经口灌胃分别给予壳聚糖和生理盐水，阳性对照组在孕期第11-13d每天腹腔注射环磷酰胺（cyclophosphamide，CP）7mg／kg，在妊娠的第20d处死大鼠进行胎鼠检查。结果：壳聚糖对孕鼠的健康状况、行为、增重和生殖机能无明显影响；对胎鼠的生长发育无明显影响；对胎鼠的外观、骨骼和内脏无致畸作用。结论：在≤1000mg／kg剂量下，壳聚糖对大鼠无胚胎毒性和致畸毒性。

BACKGROUND ＆ AIM： To study the embryonic and teratogenic toxicities of chitosan in rats during the period of organ formation. MATERIALS AND METHODS： Ninety pregnant SD rats were randomly divided into five groups with 18 rats in each, three chitosan dosage groups （50, 250, 1 000 mg/kg）, one negative and one positive control group. Chitosan and normal saline was given by garage to the treated groups and the negative control group for ten clays during the period of organ formation （the 6^th to 15^th day of gestation）, respectively. Rats in the positive control group received intraperitoneal cyclophosphamide （CP） on the 11^th to 13^th day of gestation at a daily dose of 7 mg/kg. The rats were sacrificed to examine the fetuses on the 20^th day. RESULTS： The results showed that chitosan didn＇t produce significant effects on health, behavior, weight gain and reproductive parameters of pregnant rats and fetal growth, and didn＇t induce any teratogenic effect on the appearance, bones and bowels of the fetuses. CONCLUSION： Chitosan has no embryonic and teratogenic toxicity when used under 1000 mg/kg.