紫外线照射对HaCaT细胞IL-12和IFN-γ受体表达的影响

Effects of UV on IL-12 and IFN-γ derived from HaCaT cells

目的:观察紫外线(UV)照射对HaCaT细胞起源的IL-12、IFN-γ的影响,为进一步阐述光敏性皮炎的发病机制提供依据。方法:以UVA和UVB照射人永生化表皮细胞(HaCaT细胞),应用免疫组化SP法观察UV照射后HaCaT细胞表面IL-12、IFN-γ受体的表达情况,并以阳性细胞百分比半定量IL-12、IFN-γ在HaCaT细胞中的表达;同时应用ELISA方法检测细胞培养液中IL-12、IFN-γ的含量。结果:静息状态下,HaCaT细胞低水平表达IL-12R、IFN-γR,上清液中IL-12、IFN-γ呈低水平表达或检测不出;UV照射HaCaT细胞后12 h,细胞表面IL-12R、IFN-γR及上清液中的IL-12、IFN-γ与照射前无明显差异。结论:紫外线照射后角质形成细胞产生细胞因子致T细胞亚群失衡与光敏性皮炎的相关性,有待进一步探讨。

Objective： To determine the effects of UV on IL- 12 and IFN-γ derived from HaCaT cells, in order to provide evidence in elucidating the pathogenesis of photodematitis. Methods： HaCaT cell line was irradiated by UVA and UVB in different dosage. At the time of hour 12 after irradiation, the expression of IL- 12R and IFN- γ R on HaCaT cells was measured by immunohistochemistry ochemistry （SP method） and the percentage of positive cells was calculated. At the same time, the protein levels of IL- 12 and IFN-γ in supernatant of irradiated cells was quantified with EILSA method. Results： Without irradiation, there was low expression of IL- 12R and IFN- γ R in HaCaT cells and there was no or low level of IL- 12 and IFN - γ in supernatart. The same expression of IL- 12R and IFN - γ R in HaCaT cells and in supematant was measured after irradiation. Conclusion：The correlation between unbalance of Th1/Th2 caused by UV exposure on keratinocyte and photodermatitis needs further study.