摘要
目的：探讨窄谱中波紫外线（NB—UVS）辐射对白癜风不同部位培养的黑素细胞生物学特性的影响，以指导设定NB—UVB最佳治疗间隔时间。方法：体外培养白癜风患者外观正常皮肤、皮损边缘处黑素细胞，倒置显微镜观察NB一[NB辐射对细胞形态的影响，MTT法和NaOH法检测NB—UVB辐射剂量（0-100mJ/cm^2，cm^2）和作用时间（辐射后48h、72h、96h）对细胞增殖和黑素合成的作用。结果：①NBUVB（50-mJ/cm^2）辐射黑素细胞后，细胞肿胀，树突缩短增粗，黏附性下降；②NB—UVB辐射后48h，在实验剂量下可提高细胞的黑素合成能力，辐射剂量≥60mJ／cm^2可抑制白癜风外观正常皮肤黑素细胞增殖，而≥40mJ／cm^2即可对皮损边缘处黑素细胞产生抑制作用；NB—UVB辐射后72h、96h，可促进黑素细胞增殖和黑素合成，对白癜风外观正常皮肤黑素细胞作用更明显，辐射后72h黑素细胞增殖率和黑素含量达到最高点。结论：NB—UVB辐射后72h对白癜风黑素细胞功能的影响最大，可用以指导临床设定NB-UVB照射最佳的治疗间隔时间。
Objective： To determine the effect of NB - UVB irradiation on biological characteristics of cultured mdanocytes in different sites, in order to guide optimal NB- UVB irradiation interval time. Methods： Melanocytes from both normal skin and the edge of lesion were cultured in patients with vitiligo. Effect of NB - UVB irradiation on morphology of melanocytes was observed by invert microscope. MTT method and NaOH assay were applied to detect the effect of NB - UVB irradiation dose （0- 100 mJ/cm^2 ）and action time （h 48, h 72 and h 96 after irradiation）on cell proliferation and synthesis of melanin. Results： （1） A dose of 50 mJ/cm^2 NB- UVB irradiation resulted in cellular swelling, cytodendrites shortening and thickening, and decrease in cellular adhesiveness; （2） NB- UVB irradiation enhanced the capacity of melanogenesis in a dose - dependent manner at h 48 after irradiation. Irradiation dose ≥60mJ/cm^2 inhibited proliferation of melanocytes in normal skin, while the dose ≥40mJ/cm^2 produced inhibitory action of melanocytes from the edge of lesions. NB- UVB irradiation promoted cell proliferation and melanogenesis at h 72 and h 96 after irradiation. The effect on melanocytes from natural skin was more obvious, and h 72 after NB- UVB irradiation was the time point of maximal cell proliferation rate and synthesis of melanin. Conclusion： The greatest effect on the cultured melanocytes of vitiligo occurs at h 72 after NB- UVB irradiation, which indicates the optimal NB- UVB irradiation interval time in clinical treatment of vitiligo.
出处
《中国麻风皮肤病杂志》
2007年第1期6-9,共4页
China Journal of Leprosy and Skin Diseases
