摘要
目的观察Toll样受体2/4蛋白在小鼠全肝缺血再灌注损伤中肝脏的表达,并分析其与肝功能损伤的关系。方法通过夹闭BALB/c小鼠肝门,复制小鼠全肝缺血再灌注损伤模型。采用Western blot方法定量检测缺血肝叶中TLR2/4蛋白的表达变化,并检测门静脉血浆丙氨酸氨基转移酶(pALT)、肿瘤坏死因-α(TNF-α)及门静脉血清内毒素(endotoxin,EN)水平。结果与假手术组(sham-operated group,SH组)相比:(1)全肝缺血20min并行再灌注后,缺血再灌注组(ischemic/reperfusion group,I/R组)血清ALT在再灌注1h即明显升高,且在再灌注3h时较1h时明显升高;(2)I/R组缺血肝脏TLR2/4蛋白的表达(OD值)明显升高,TLR2蛋白的表达在再灌注3h较1h明显高;而TLR4蛋白的表达以再灌注1h时水平最高。(3)I/R组中门静脉血清TNF-α在再灌注1h即开始高,在再灌注3h达高峰。(4)门静脉血清内毒素水平明显升高(与SH相比,P〈0.01),但I/R组在不同灌注时间点之间无显著性差异(P〉0.05)。结论TLR2/4蛋白表达的上调参与了小鼠全肝脏缺血再灌注中肝脏的损伤。
Objective To investigate the expression of TLR2/4 protein in mouse total hepatic ischemia/reperfusion (I/R) injury process and explore its relation to liver functional damage. Methods BALB/c mice were used to establish the model of total hepatic I/R injury by obstructing the portal vein. The changes of TLR2/4 protein in ischemic liver lobes were determined by Western blot. Meanwhile, the levels of plasma ALT, endotoxin and serum TNF-α in portal vein were measured. Results 1) After 20 minutes of total liver ischemia, the AST level in I/R group was significantly increased as compared with the sham-operated (SH) group, and AST level at the 3rd reperfusion was markedly higher than that at the 1st reperfusion (P〈0. 01). 2) The expression of TLR2/4 protein was remarkably up-regulated in I/R group than in the SH group at the time point of 1st and 3rd h reperiusion. The expression of TR2 protein was higher at 3rd h reperfusion than that at the 1st h. TLR4 peaked at the 1st h. 3) the level of portal vein TNF-α significantly increased in the I/R group, and the data at the 3rd h reperiusion were higher than those at the 1st h. 4) Endotoxin in the portal vein was markedly higher in I/R group than in the SH group (P〈0. 01). However, there was no difference at the 1st h and 3rd h reperfusion (P〉0. 05). Conclusions The up-regulation of TLR2/4 protein in mouse total hepatic I/R injury process might be involved in the process of liver injury.
出处
《中华肝胆外科杂志》
CAS
CSCD
2007年第1期45-47,共3页
Chinese Journal of Hepatobiliary Surgery
基金
国家自然科学基金资助项目(30200272)