抗CD81抗体对星形胶质细胞增殖的抑制作用

THE INHIBITION EFFECT OF ANTI-CD81 ON THE PROLIFERATION OF ASTROCYTES

目的研究抗CD81抗体在星形胶质细胞增殖中的作用。方法将纯化的星形胶质细胞分为6组,加入不同浓度抗CD81抗体,其浓度依次为0、0.1mg/L、0.5mg/L、1mg/L、5mg/L、10mg/L,以四甲基噻唑蓝(MTT)比色法检测细胞活性。在此检测结果的基础上,选出3个有意义的浓度组,加入浓度分别为0、0.5mg/L、5mg/L的抗CD81抗体,采用流式细胞术观察抗CD81抗体对星形胶质细胞周期的影响并进行统计学分析。结果抗CD81抗体对星形胶质细胞的增殖有抑制作用,并呈一定的剂量依赖性。加入抗CD81抗体培养24 h后,实验组的星形胶质细胞处于G0/G1期的细胞指数减少,S期细胞指数增多。结论抗CD81抗体抑制了星形胶质细胞的增殖,使星形胶质细胞的细胞周期受阻,阻滞于S期。

Objective To investigate the effect of anti-CD81（antibodys against CD81） on the proliferation of astrocytes. Methods Purified astroeytes from newborn rats＇ cerebral cortex were divided into 6 groups and added with anti-CD81 different concentrations（0,0.1,0.5,1,5, 10 mg/L）. The activity of astrocytes was tested by methyl thiazolyl terazolium（MTT）. Three significative groups were chosen based on MTT result and added with anti-CD81 of different concentrations（0,0.5,5mg/L）. After administration for 24 hours, the cell cycle of the astrocytes was measured by flow eytometer. The corresponding data were analyzed with SPSS statistical software. Results 1 .By MTr, the average optical density（AOD） values of astrocytes were reduced after administration with anti-CD81 of different concentrations for 24 hours, that is, the number of astrocytes was reduced, which indicated anti-CD81 inhibited the proliferation of astrocytes and the effect showed a dose-dependent pattern. 2. By cell cycle analysis, a progressive dose-dependent decrease was found in the index of cells in G0/G1 phase and an increase in S phase. Such as, the index of cells in G0/G1 phase, was 82.73 in 0, is 82.16 in 0.5 mg/L, was 78.58 in 5 mg/L.Conclusion Anti-CDS1 inhibits the proliferation of astrocytes and the number of astroeytes is reduced. Further more, the index of cells decreases in G0/G1 phase and increases in phase S after administration with anti-CD81. This study shows that anti-CD81 doesn＇t restrain the cells from G1 phase to S phase but the cells are arrested in S phase.