电针刺激内关穴对心肌缺血再灌注损伤家兔心肌细胞凋亡调控基因的影响

The effects of electro-acupuncture at Neiguan point on myocardial apoptosis controlling genes in the rabbit models of myocardial ischemia and reperfusion injury

目的探讨电针刺激内关穴对心肌缺血再灌注损伤家兔心肌细胞凋亡调控基因Bax和Bcl-2的影响。方法将36只家兔随机分为假手术组、心肌缺血再灌注组（模型组）、电针刺激内关穴组（关穴组）和电针刺激列缺穴组（列缺组）。采用结扎左冠状动脉前降支30min再灌注60min的方法，建立心肌缺血再灌注损伤模型。关穴组和缺穴组分别采用电针刺激实验动物双侧内关穴和列缺穴。应用免疫组织化学法观察电针对缺血再灌注损伤家兔心肌细胞凋亡调控基因Bax和Bcl-2的影响。结果模型组家兔心肌Bax呈高表达，而内关组心肌Bax表达显著降低（P〈0．01）；内关组与模型组和列缺组比较，心肌Bcl-2表达显著增加（P〈0．01或0．05）。结论电针刺激内关穴能抑制心肌Bax的表达，促进Bcl-2的表达，从而抑制缺血再灌注后细胞凋亡的发生，对心肌细胞起到保护作用。

Objective To study the effects of electro-acupuncture （EA） on myocardial apoptosis controlling genes （Bax, Bcl-2） in the rabbit models of myocardial ischemia and reperfusion injury （M1R1）. Methods Thirtysix rabbits were randomly divided into a sham-operation group, a model group, an EA at Neiguan group （N group） and an EA at Lieque group （ L group）. The M1R1 models were established with ligation of the left anterior descending coronary artery lasting for 30 min and followed by reperfusion for 60 min, and rabbits in groups N and L were treated with EA at Neiguan or Lieque, respectively. Then the expression of Bax and Bcl-2 was measured by using immunohistochemical technique. Results The Bax was over-expressed in the model group, but significantly decreased in the N group （P 〈 0.01 ）. The expression of Bcl-2 was markedly increased in the N group with comparison to that in the model and L groups （ P 〈 0.01, P 〈 0.05 ）. Conclusion EA at Neiguan has protective effects on cardiac musale cells by inhibiting the expression of Bax and increasing the expression of Bel-2.