高压氧对缺血再灌注损伤小鼠脑区甘丙肽含量的影响

The effects of hyperbaric oxygen therapy on the concentrations of galanin in brain areas of mice with ischemical reperfusion injury

目的观察小鼠缺血再灌注损伤后各脑区甘丙肽（Gal）含量的变化以及高压氧（HBO）治疗对其含量的影响。方法昆明小鼠24只，随机分为缺血再灌注＋高压氧（HBO）组（A组）、缺血再灌注组（B组），假手术组（C组）和正常对照组（D组），每组6只。A、B组动物采用阻断双侧颈总动脉血流30min后恢复血流的方法建立脑缺血再灌注模型。采用放射免疫分析法测定各组动物脑区Gal免疫活性物质（ir-Gal）含量的变化。结果小鼠缺血再灌注损伤后各脑区Gal含量均有不同程度的变化，皮质ir-Gal含量明显升高，海马ir-Gal含量却明显下降，与C组和D组比较差异均有统计学意义；纹状体ir-Gal含量与D组接近（P〉0．05）。HBO处理后，海马ir-Gal含量明显提高并接近正常水平，皮质与纹状体Gal含量在HBO处理后也明显高于B组和C组，差异均有统计学意义。结论Gal参与了缺血再灌注损伤的病理生理过程，不同脑区的Gal在缺血再灌注损伤中的重要性及作用可能有所不同。HBO处理后各脑区Gal含量明显提高，可能是减少缺血再灌注损伤的作用机制之一。

Objective To explore the effects of hyperbaric oxygen therapy （HBO） on the concentrations of galanin of brain areas in mice with cerebral ischemic reperfusion injury （CIRI）. Methods Twenty-four Kunming mice were randomly and evenly divided into an ischemic reperfusion ＋ HBO group （group A） , an ischemic reperfusion group （group B） , a sham operation group （group C） and a normal group （group D）. CIRI models were established by using the common carotid arteries occlusion for 30 min and post-ischemic reperfusion. HBO treatment was employed after the establishment of the models once a day for 10 d, then the quantification of galanin immunoreactivity （ir-Gal） was measured with radioimmunoassay. Results Compared with those in groups C or D, the concentrations of ir-Gal were significantly elevated in cortex but lowered in hippocampus in group A. Whereas there was no significant difference in striatum in group A with comparison to that of group D. After HBO treatment, the descent of ir- Gal in hippocampus was attenuated, and ir-Gal levels were significantly increased in cortex and striatum with comparison to those in groups B and C （P 〉 0.05）. Conclusion Galanin plays an important role in the CIRI pathophysiologic process with different effects in different brain areas. The increase of ir-Gal after HBO treatment might be a potential mechanism of HBO in alleviating CIRI.