摘要
目的研究FasL基因转染树突状细胞(DC)对屋尘螨致敏/激发小鼠气道炎症的影响。方法将FasL基因转染的DC和对照DC(未转染DC)分别经气管注入屋尘螨致敏/激发小鼠体内,病理切片观察小鼠肺部炎症,取支气管肺泡灌洗液(BALF),行BALF细胞总数及分类计数,ELISA检测BALF的白细胞介素-4(IL-4)、IL-5和γ干扰素(IFN-γ)水平。结果FasL基因转染的DC过继处理屋尘螨致敏/激发小鼠,能显著地减轻致敏/激发小鼠气道变应性炎症,减少BALF中细胞总数和嗜酸粒细胞比例,降低BALF中IL-4和IL-5的水平,增高IFN-γ水平。结论FasL基因转染的DC过继处理屋尘螨致敏/激发小鼠能抑制Th2细胞活化,抑制变应性气道炎症。
Objective To investigate the effects of FasL gene-modified dendritic cell （DC） on the airway inflammation in mice sensitized/challenged by house dust mite （HDM） allergen. Methods FasL genemodified DC （FasL-DC） and control DC （nontransfection DC） were administrated into HDM sensitized and challenged mice by intratracheal injection respectively, then HDM sensitized and challenged mice were sacreificed two days later. Total and differentiation cell counts and levels of interleukin-4（IL-4）, IL-5 and interferon-γ （IFN-γ） in bronchoalvcolar lavage fluid （BALF） were detected and lung histological features were observed. Results After administration of FasL-DC,lung allergic inflammation was ameliorated while total cell counts,the percentage of eosinophil, the levels of IL-4 and IL-5 in BALF decreased and the level of IFN-γ in BALF increased. Conclusion Administrating FasL-DC into HDM seusitized/challenged mice can inhibit Th2 cells activation and ameliorate airway allergic inflammation.
出处
《中国呼吸与危重监护杂志》
CAS
2007年第1期15-18,共4页
Chinese Journal of Respiratory and Critical Care Medicine
基金
国家自然科学基金(编号:30470772)
