摘要
目的:观察榄香烯乳联合足叶乙甙对视网膜母细胞瘤HXO-RB44细胞凋亡及survivin表达的影响及其作用机制。方法:采用榄香烯乳、足叶乙甙单药及两者联合处理视网膜母细胞瘤HXO-RB44细胞,观察HXO-RB44细胞生长情况并采用四甲基偶氮唑蓝(MT)结果:HXO-RB44细胞经榄香烯乳、足叶乙甙及两者联合处理后,细胞增殖受到不同程度的抑制(r=0.963,P<0.05),survivin蛋白表达下降(P<0.01);其中以两者联合用药最为显著。结论:榄香烯乳联合足叶乙甙能协同抑制HXO-RB44细胞的增殖,并可能下调survivin的表达,促进细胞的凋亡,抑制RB细胞系增生。
AIM： To investigate the effects of elemene and/or etoposide on the apoptosis of human retinoblastoma HRO-RB44 cell and the expression of survivin in vitro.
METHODS： The HRO-RB44 cells were treared with elemene and/or etoposide for 24 hours, and then the proliferation was assessed by MTT method. The survivin expression were analyzed by flow cytometry and RT-PCR.
RESULTS： Elemene and etoposide could inhibit the growth of HXO-RB44 cells, down-regulate the expression of survivin, and induce extensive apoptosis of HRO-RB44 cell.
CONCLUSION： Elemene could enhance the effects of etoposide on proliferative suppression, survivin expression down-regulation, and apoptosis induction of HRO-RB44 cells.
出处
《国际眼科杂志》
CAS
2007年第1期64-66,共3页
International Eye Science
