体外构建含细胞组织工程角膜基质的方法

In vitro construction of cellular tissue engineering corneal stroma

目的:研究体外构建组织工程角膜基质的方法。方法:用去垢剂联合酶的方法脱细胞处理猪角膜基质,在去细胞猪角膜基质材料上接种体外培养的兔角膜基质细胞,并将兔角膜基质细胞进行PKH26荧光标记,在体外构建组织工程角膜基质。用倒置荧光显微镜;HE染色光学显微镜;扫描电镜观察。结果:利用异种去细胞角膜基质组织为支架材料而构建的组织工程角膜基质具有近似正常角膜基质的三维立体结构。结论:以去细胞猪角膜基质材料为支架,利用组织工程技术可成功的在体外构建出含细胞的组织工程角膜基质组织。

AIM： To study the in vitro construction process of cellular tissue engineering cornea stroma. METHODS： Porcine cornea sb＇omata were processed by serial digestion of trypsin, Dnase and Rnase. Then rabbit corneal stroma cells were raised in it, marked by fluorchrome pkh26. Tissue engineering cornea stromata were constructed in vitro. Inverted fluorescence microscope, HE staining light microscope and scanning electron microscope were used in the observation. RESULTS： In vitro constructed tissue engineering cornea stroma had the similar three diamension construction with the normal corneal stroma. Conclusion： Acellular porcine cornea stroma materials can be used as carrier material to construct tissue engineering cornea in vitro.