高效液相色谱法测定人血浆罗哌卡因的浓度

Determination of the concentration of ropivacaine in patients by high performance liquid chromatography

目的 建立测定人血浆罗哌卡因浓度的高效液相色谱方法。方法 LC-6A高效液相色谱仪，色谱柱为Hy—persilC18（150mm×4．6mm，5μm）。以布比卡因为内标，流动相采用乙腈：水=17：83（V／V），流速2．0ml／min，在紫外检测波长210nm处进行检测。结果 本方法标准曲线线性方程为Y=1．1236X＋0．0245（y=0．9997，线性浓度范围为0．025～2．5mg／L）；最低检测限为0．0125mg／L；平均方法回收率为99．63％；日间、日内精密度RSD均〈9％。结论 本方法简便、灵敏、快捷、准确，可用于临床上罗哌卡因血药浓度的监测和药代动力学研究。

Objective To establish a high performance liquid chromatography （HPLC） method for determination of the concentration of ropivacaine in human plasma. Methods LC - 6A HPLC instrument was used with the column of Hypersil C18（ 150 mm ×4.6 mm,5μm）. Internal standard was bupivacaine. The mobile phase composed of acetonitrile -water was 17：83 （V/V）. The flow rate was 2.0 ml/min. The fluorescence detective wave length was 210 nm. Results The standard curve equation was Y = 1. 1236X ＋ 0. 0245 （ y = 0. 9997）. The linear range was 0. 025 -2. 5 mg/L. The limit of detection was 0. 0125 mg/L. The average recovery was 99.63%. RSD of inter - day and intra - day precision（ RSD% ） were all less than 9%. Conclusion The HPLC method is simple, accurate and sensitive for monitoring ropivacaine concentration in clinical plasma samples and pharmacokinetics study.