银杏叶提取物对大鼠肝脏缺血再灌注损伤的保护作用

Protective effect of ginkgo biloba leaves extract on the damage of ischemia reperfusion injury in rat liver

目的 研究银杏叶提取物对大鼠急性肝脏缺血再灌注损伤的保护作用及其机制。方法 SD大鼠随机分为假手术对照组、单纯缺血再灌注组、缺血再灌注＋银杏叶提取物处理组。通过阻断大鼠肝门30min后再开放建立肝缺血再灌注损伤模型，在肝脏再灌注90min时测肝组织丙二醛（MDA）、超氧化物歧化酶（SOD）和测血ALT、AST，并观察肝组织病理学改变。结果再灌注90min时缺血再灌注（1／R）＋银杏叶提取物处理组的肝组织MDA生成，SOD消耗，血清ALT、AsT升高值均少于L／R组（P〈0．01），且L／R＋银杏叶提取物处理组的肝细胞显微结构损害的改变较L／R组轻。结论 银杏叶提取物通过抗氧化，对急性肝脏缺血再灌注损伤有保护作用。

Objective To study the preventive effect of damage of ischemia reperfusion in rat liver by applying ginkgo biloba leaves extract（EGb） injection. Methods SD rats were divided randomly into sham operated control （SOM） group, ischemia reperfusion （I/R） group and ischemia reperfusion ＋ ginkgo biloba leaves extract （EGb） （ I/R ＋ EGb） group. The animal model of hepatic ischemia reperfusion was made by temporarily blocking the hepatic portal blood vessels and releasing of the hepatic portal occlusion was carried out 30 minutes later. The malondialdehyde （MDA）, superoxide dismutase （SOD） in liver tissue and AST, ALT levels in the blood were determined after the liver was reperfuded for 90 minutes, the hepatic tissue was examined under light and microscopies. Results Increase of MDA, AST, ALT and decrease of SOD of I/R group were found significantly as compared with those in the I/R ＋ EGb group （ P 〈 0. 01 ）. The histologic changes of the liver tissue under light microscopies in the I/R group were more obvious than those in the I/R ＋ EGb group. Conclusion Ginkgo biloba leaves extract has the ability to scavenge oxygen free radical （OFR） and to protect from the ischemia reperfusion injury in rat liver.