影响农杆菌渗入法瞬间表达重组蛋白系统的若干因素

Factors Influencing the Agroinfiltration System of Transient Expression of Recombinant Protein

利用农杆菌渗入法(Agroinfiltration)在植物中瞬间表达重组蛋白的系统,具有高效、安全、简便的特点,是一种重要的生产重组蛋白的方法。概述了Agroinfiltration系统的建立、发展及在生产重组蛋白领域的应用进展;并对影响该系统的技术因素作了深入探讨。

The plant-based transient expression system using Agroinfiltration is high-efficient,safe and simple,which appears to be a promising technology for producing recombinant proteins. In this paper,we first summarize the establishment and the development of Agroinfiltration and its application in the aspect of producing recombinant proteins, and then some technical factors influencing the system are deeply discussed.