珍珠菜总黄酮苷诱导HL-60细胞凋亡作用的研究

Study on Apoptosis of HL-60 Cell Line Induced by Total Flavones of Lysimachia Clethroides Duby

目的:对珍珠菜总黄酮苷(ZTF)诱导人白血病HL-60细胞凋亡作用进行研究。方法:HL-60细胞经ZTF作用后,通过光镜和透射电镜观察细胞的形态学变化,采用流式细胞仪和琼脂糖凝胶电泳观察ZTF对HL-60细胞周期的影响。结果:HL-60细胞经ZTF作用后,形态学和琼脂糖凝胶电泳试验均可观察到明显的细胞凋亡形态;流式细胞仪测定结果表明作用24h,可使HL-60细胞凋亡,作用48h后,可使HL-60细胞阻滞于G2/M期。结论:ZTF可能直接损伤肿瘤细胞DNA及干扰细胞某些蛋白的合成。

Objective：To investigate the apoptesls of HL-60 cells induced by total flavones of lysimachia clethroides duby （ZTF）. Methods：HL-60 cells were treated with ZTF, and then morphologi＇c changes under fight microscope and electron microscope were oh- served：alternation of cell cycle was analyzed by flow cytometry and agarose gel test.Results：After treated with ZTF,typical morphology characters of apoptesis could be found in HL-60 cells through morphologic and agarose gel test：the results of flow cytometry showed that ZTF could induce apoptesis in HL-60 cells after 24h,and HL-60 cells were blocked in the G2/M of cell cycle after treated with ZTF 48 h later. Conclusion： ZTF can directly damage DNA of cancer cells and interfere in the synthesis of some proteins in the cells,