摘要
目的探讨特异性免疫荧光法在痰标本结核分枝杆菌检查上应用的可行性。方法制备高效价结核分枝杆菌免疫血清和单克隆抗体,并用异硫氰酸荧光素(FITC)标记,痰标本涂片、固定,加荧光抗体结合,荧光显微镜下检查。结果60份临床疑似肺结核病人痰标本,用抗酸染色法、结核分枝杆菌免疫血清和单克隆抗体免疫荧光法三种方法检测,阳性率分别为:36.7%(22/60)、58.3%(35/60)和56.7%(34/60),其中,抗酸染色阳性22份标本,两种免疫荧光法检查均为阳性,吻合率100%。结论免疫荧光法检查痰标本结核分枝杆菌阳性率大大高于抗酸染色,进一步完善后可尝试用于痰标本的结核分枝杆菌检查。
Objective To probe the feasibility with specific inanunofluorescence technique to determine the sputum M - tuber- culosis. Methods The high- fiter M- TB immuno- serums and monodonal antibody was made, marked by fluorescein isothio- cyanate （FTTC）. Sputum was smeared and firmed then to add fluorescence antibody, observed with fluorescence microecope. Results There were 60 sputum of clinical suspect pulmonary tuberculosis cases in all were detected with methods of acidfast stain and specific inmaunofluorescence （complex antibody and Mc- antibody）. The positive- rate of M- tuberculosis were 36.7% （22/60）, 58.3%（35/60）and 56.7% （34/60） respectively.Among these detecting, positive of sputums with acid- fast stain were all positive in immunofluorescence tests, and the identical- rate was 100%. Conclusion Imnunoiluorescent test was used in the determine of sputurns M - TB had more positive - rate than classical acid - fast stain.After further improvement, it is necessary to try to use to determine of sputums M- tuberculosis.
出处
《中国预防医学杂志》
CAS
2007年第1期9-11,共3页
Chinese Preventive Medicine
基金
国家自然科学基金资助项目(30471526)
