葡萄糖氧化酶膜过滤发酵工艺的研究

Study on Membrane Filtration Fermentation for Glucose Oxidase

在葡萄糖氧化酶发酵过程中，配以外置式的膜过滤系统进行膜过滤发酵，可减缓发酵过程中产物及分解代谢物对酶合成的阻遏和抑制作用，使酶产率增加。实验结果表明：膜过滤发酵的产酶速率高达４．１４０×１６．６７μｍｏｌ／ｓ·ｈ（４１４０．１９Ｕ／ｈ），比分批发酵高３倍以上，且能在较长时间内维持较高的酶产率。膜过滤发酵过程中ｐＨ、ＮＨ＿２－Ｎ和葡萄糖酸的含量随补糖速率和稀释率Ｄ的大小发生变化，发酵液中葡萄糖含量控制在２．０％～２．５％，Ｄ控制在０．１２ｈ￣（－１）为宜。

Glucose oxidase(GOD)fermentation in stirred tank bioreactor coupled with a membrane filtration system is evaluated in order to remove the catabolites inhbition and repression and to increase the enzyme productivity.Experimental results show that the maximum enzyme productivity is 4.14×16. 6μmol/s·h(4140.19U/ h),which is 3 times higher than that of the batch fermentation.The operation conditions for membrane filtration are also studied.The optimum glucose concentration in broth is controlled within 2.0% ～2.5%,The optimum dilution rate is 0.12h～(-1).