RhoC反义基因转染对人胆管癌QBC939细胞增殖和侵袭性的影响

Effect of antisense RhoC gene on the proliferation and invasion abilities of human QBC939 cholangiocarcinoma cells

目的研究RhoC反义基因对胆管癌QBC939细胞株增殖和侵袭的影响。方法以脂质体将反义RhoC cDNA真核表达载体转染人胆管癌QBC939细胞。应用克隆形成试验检测细胞增殖活性的变化，流式细胞仪检测转染前后细胞周期的变化，Transwell小室检测侵袭能力的变化。结果转染反义RhoC cDNA真核表达载体后，形成克隆数目较空载体转染细胞和未转染的QBC939细胞明显减少[（54±8）VS（91±11）VS（90±9），P〈0．05]；并出现G1期阻滞（52．5％vs43．4％vs43．7％）；穿过小室滤膜细胞数目明显减少[（36±6）V8（96±12）V8（95±7），P〈0．05]。结论RhoC反义基因能够抑制胆管癌QBC939细胞株的体外增殖和侵袭力。

Objective To explore the antisense RhoC gene on the proliferation and invasion properties of cholangiocarcinoma cell line （QBC939）. Methods The antisense RhoC cDNA was transfected into human cholangiocarcinoma cell line （QBC939） with Lipofectane 2000. The cell growth curve was made to determine the inhibitory rate of cells ; Flow cytometry was used to analyze the changes of cell cycle of the tumor cells; Boyden chamber was made to detect the invasive ability of cells before and after the gene transfection. Results After the antisense RhoC cDNA was transfected, the number of clone formation was significantly less （54 ± 8 vs 91 ± 11 vs 90 ± 9,P 〈 0. 05）, the number of cells which penetrated the lower surface of the matrigel-coater filters was also less （ 36 ± 6 vs 96 ± 12 vs 95 ± 7, P 〈 0. 05 ）, and percentage of cells in G1 was higher （52.5% vs 43.4% vs 43.7% ） than in vacant vector transfected cells and non-transfected cells. Conclusion Antisense RhoC gene could suppress the abilities of proliferation and invasion in cholangiocareinoma cell line in vitro.