犬双肺序贯性移植的研究

The experimental study of canines sequential bilateral lung transplantation

目的探讨犬双肺序贯性移植时供肺的采取及受体移植手术技巧。方法犬16条，体重差异〈5％的随机配对作为供受体进行双肺序贯移植实验共8次，移植右肺钳夹左房时采用了‘v’钳夹技术，并检测左肺及右肺术后0．5、1、2h各进行血气分析、肺水含量及电镜下超微结构，了解术后肺功能。结果移植右肺时采取的‘v’型钳夹术良好地解决了钳夹左房时因回心血量受阻而引起的心功能不全。术后检测结果表明移植后肺功能良好。结论掌握了犬双肺序贯性移植的全过程，“V”形双阻断钳夹左心房技术提高了犬肺移植的成功率。

Objective To investigate the techniques of the donor lungs harvest and recepient lung transplantation in canines sequential bilateral lung. Methods Sixteen canines were divided into eight groups ramdonly. In each group, the weight ranges varied within 5 percents. Improved clamp technique were used when transplanted right lung. Blood gas analysis, water content of the lung tissue and electron microscope were used to evaluate the lung function after operation. Results The technique of ‘v＇ shape clamp could improve left heart function, which caused by decreased returned blood volume when left atrium was clamped. The postoperatlonal test results indicated that the transplanted lung function were satisfactory. Conclusions The whole process of canines sequential bilateral lungs transplantation were obtained, ＂V＂ shape double-block technique could increase success ration of canines lungs transplantation.