摘要
目的:研究线粒体prohibitin蛋白在紫杉醇耐药的CCRF-CEM细胞中的表达变化,探讨线粒体prohib-itin蛋白在CCRF-CEM肿瘤细胞耐药发生机制中的作用。方法:提取紫杉醇耐药的CCRF-CEM/taxol细胞的线粒体总蛋白,对经双向电泳分离后得到的SDS-PAGE胶用ImageMasterTM2D Platinum software进行分析、比对,对胶上的蛋白点进行质谱分析,通过SWISS-PROT和NCBInr数据库鉴定蛋白的归属。结果:紫杉醇耐药的CCRF-CEM/taxol细胞中的线粒体prohibitin Protein15表达升高了68.6%(以蛋白表达的体积计)。结论:紫杉醇耐药的CCRF-CEM/taxol细胞中线粒体prohibitin表达增加。prohibitin可能通过调节线粒体的活动,对抗紫杉醇诱导的细胞凋亡,在耐药机制中发挥重要作用。
Objective： To elucidate the role of mitochondrial prohibitin in multi-drug resistant CCRF-CEM cells by determination of expression status of the prohibitin. Methods： The total mitochondrial proteins from CCRF-CEM cells or paclitaxol-resistant CCRF-CEM cells were extracted. The isolated proteins were separated by two-dimensional electrophoresis and then analyzed using ImageMasterTM 2D Platinum software. The protein spots on the gel were analyzed by MS and identified by comparison to SWISS-PROT database and NCBInr database. Results： One of the two mitochondrial prohibitins was overexpressed in CCRF-CEM/paclitaxol by 68.6% （ per protein volume expressed） compared with that in CCRF-CEM cells. Conclusion： The mitochondrial prohibitin may reduce multidrug resistances against the paclitaxol-induced CCRF-CEM cellular apoptosis by regulation of the mitochondria activities.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2007年第1期40-45,共6页
Chinese Journal of New Drugs
