异丙酚对大鼠肝脏缺血再灌注损伤的保护作用

Protective effect of propofol on liver ischemia-reperfusion injury in rats

目的探讨异丙酚对大鼠肝脏缺血再灌注的保护作用及其机制。方法Ⅰ组:假手术组;Ⅱ组:模型对照组,经尾静脉持续输注0.9%NS1ml/kg.h;Ⅲ组:异丙酚5mg/kg.h组;Ⅳ组:异丙酚10mg/kg.h组;Ⅴ组:异丙酚20mg/kg.h。分别在肝门阻断30min、再灌注60min取腹主动脉血,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性。测定肝组织丙二醛(MDA)含量、黄嘌呤氧化酶(XOD)、超氧化物岐化酶(SOD)活性,并电镜观察肝组织超微结构。结果Ⅱ、Ⅲ、Ⅳ、Ⅴ组血清ALT、AST活性均高于Ⅰ组(P<0.01),Ⅲ、Ⅳ、Ⅴ组血清ALT、AST活性均低于Ⅱ组(P<0.05或0.01),Ⅲ、Ⅳ、Ⅴ组间血清ALT、AST活性差异具有显著性(P<0.05或0.01)。与Ⅰ组比较,Ⅱ、Ⅲ、Ⅳ、Ⅴ组肝组织MDA含量升高、XOD活性升高(P<0.05或0.01),Ⅱ组肝组织SOD活性降低,Ⅲ、Ⅳ、Ⅴ组肝组织SOD活性升高;与Ⅱ组比较,Ⅲ、Ⅳ、Ⅴ组肝组织MDA含量降低、XOD活性降低、SOD活性升高(P<0.05或0.01)。Ⅲ、Ⅳ、Ⅴ组间肝组织MDA含量、XOD活性、SOD活性差异亦具有显著性(P<0.05或0.01)。Ⅲ、Ⅳ、Ⅴ组肝细胞病理学改变轻于Ⅱ组,且Ⅲ、Ⅳ、Ⅴ组间肝细胞病理学改变有差异。结论异丙酚对大鼠肝脏缺血再灌注损伤中具有明显保护作用,与其抗氧化性有关。

Objective To investigate the protective effect of propofol on the liver against ischemia-reperfusion （ I/ R） injury and the mechanisms involved. Methods Group I was sham operation ; group Ⅱ ： 0. 9% NS was infused at 1 ml/kg · h staring from 20 min before isehemia until hepatic hilum was clamped ; group Ⅲ ： propofol 5 mg/kg · h;groupiv ： propofol 10 mg/kg · h;groupⅤ ： propofol 20 mg/kg · h. Liver I/R was produced by clamping the hepatic hilum for 30 min, then the clamp was removed for 60 min reperfusion. Blood samples were taken at the end of 60min reperfusion for determination of ALT, AST activities and meanwhile a liver specimen was obtained for determination of MDA, XOD and SOD activities and for electron microscopic examination. Results Serum ALT and AST activities were signifi ly lower in group Ⅲ ferent in group Ⅲ, 1V group Ⅱ , Ⅲ, Ⅳ and cantly higher in group Ⅱ , Ⅲ, Ⅳ and Vthan that in group Ⅰ （P 〈0. 01 ） ,but were significantly lower in groupⅢ, Ⅳ and Ⅴ than in group Ⅱ （ P 〈 0. 05 ）. Serum ALT and AST activities were significantly different in group Ⅲand Ⅴ（P 〈0.05）. V than in group Ⅰ , The MDA content and XOD activity of liver were significantly higher in group Ⅱ,Ⅲ,Ⅳand Ⅴ than in groupⅠ ,but were significant lower in group Ⅱ,Ⅲ,Ⅳ and than in group Ⅱ （P 〈 0. 05）. The SOD activity of liver was significant lower in group Ⅱ than in group Ⅰ , but significant higher group Ⅲ,Ⅳ and V than in group Ⅰ. The MDA content and XOD activity of liver were significantly higher in group Ⅲ,Ⅳ and V than in group II , and SOD activity of liver was significant lower in group Ⅲ,Ⅳ and V than in group Ⅱ （ P 〈 0. 05）. Whereas the MDA content, XOD and SOD activity of liver were also significantly different in group Ⅲ,Ⅳ and Ⅴ （ P 〈 0. 05）. Electron microscopic examination showed that the pathologic changes induced by ischemia-reperfusion were slight in group Ⅲ,ⅣandⅤ as compared with group Ⅱ , and had difference between group Ⅲ,Ⅳ andⅤ. Conclusion Propofol has significant protective effect on the rats liver against ischemia-reperfusion injury,which is related with its antioxidation.