东北林区鼠类粒细胞埃立克体感染的调查

Investigation on Anaplasma phagocytophilum infection in rodents from forest areas in northeastern China

目的了解东北林区啮齿动物感染粒细胞埃立克体病原体的情况。方法运用聚合酶链反应方法对吉林、黑龙江、内蒙古林区采集的啮齿动物标本粒细胞埃立克体的16S rRNA和gltA基因片段进行检测并测序，将所测序列与OenBank中注册的基因序列进行比较分析。结果共检测鼠标本276份，其中吉林长白山林区102份，黑龙江小兴安岭林区61份，内蒙古大兴安岭林区113份，阳性率分别为8．82％、1．64％及0．00％。体表有寄生蜱的鼠感染危险性是体表无寄生蜱鼠的11.30倍（P=0．002）。吉林、黑龙江林区鼠标本及其寄生蜱标本16SrRNA基因序列完全相同，与美国、瑞典、日本等国家检出的粒细胞埃立克体对应序列的同源性为97％～99％。gltA基因核苷酸序列与GenBank注册的相应片段比较相似性为87％～97％，推导的氨基酸序列相似性为84％～99％。结论吉林及黑龙江林区存在粒细胞埃立克体宿主动物感染。

Objective To investigate the prevalence of Anaplasma phagocytophilum in rodents from forest areas in northeastern China. Methods PCR amplification, followed by sequence analysis was carried out. The sequences of 16S rRNA and gltA gene fragment amplified from rodent specimens were compared with corresponding part of the sequences deposited in G-enBank. Results A total number of 276 rodents were tested, including 102 in Jilin province, 61 in Helongjiang province and 113 in Inner Mongolia autonomous region. The positive rates were 8.82 %, 1. 64 % and 0.00 %, respectively. The infection rate in rodents infected by ticks was 11.30 times higher than that in rodents without ticks（ P= 0.002）. The A. phagocytophilum 16S rRNA sequences from rodents in Jilin and Heilongjiang were identical and differed in 3-5 bases compared with the corresponding parts of A. phagocytophilum from America, Sweden and Japan. Compared with the sequences registered in GenBank, the nucleotide sequence of gltA varied from 87%-97% and its deduced amino acid sequence changed from 84% 99%. Conclusion A. phagocytophilum infection was presented in rodents from Jilin and Heilongjiang province.