珍稀药用真菌——樟芝深层发酵培养条件的优化

Optimum of Submerged Fermentation Parameters for Antrodia camphorata

对樟芝深层发酵培养基进行了筛选,并在此基础上对发酵条件进行了优化。以樟芝深层发酵菌丝体三萜产量为主要目标产物,确定发酵培养条件为:40g/L葡萄糖,6g/L豆饼粉,1g/L K_2HPO_4,0．5g/L MgSO4,VB_1 100mg/L,自然pH,接种量为20%,装液量为100mL/250mL三角瓶,转速100r/min,26℃恒温培养6d,胞内三萜产量达15．25mg/100mL发酵液。

Optimal parameters of submerged fermentation for Antrodia camphorata were studied. According to the yield of Intracellular triterpene, the optimal fermentation parameters were obtained as follows： 40g/Lglucose, 6 g/L soybean, 1 g/L K2HPO4, 0. 5 g/L MgSO4 and 100mg/L VBI . The optimum cultural period was 6 d at 26℃ in 250mL shake flasks at 100r/min. The best aeration ratio was 1 ： 2.5 （ medium volume： flask volume） . The inoculation volume was 20%. The intracellular triterpene of Antrodia camphorata was obtained （about 15.25mg/100mL） in the optimum culture.