血清和Pluronic　F68对悬浮培养杂交瘤细胞的保护作用

Protective Mechanism of Serum and Pluronic F68 on Hybridoma Cells in Suspended Cultures

报道了生物反应器中血清和ＰｌｕｒｏｎｉｃＦ６８对杂交瘤细胞的保护作用及其物理机理。实验发现当生物反应器中流体主体Ｋｏｌｍｏｇｏｒｏｖ湍流涡旋尺度与细胞大小相当或小于细胞时，血清和ＰｌｕｒｏｎｉｃＦ６８未能对细胞提供保护作用。而当气泡是导致细胞破损的主要根源时，它们对细胞的保护作用均十分显著，尤其是ＰｌｕｒｏｎｉｃＦ６８，在其浓度为１ｇ／Ｌ时，完全防止了气泡对细胞的破损作用。通过泡沫分馏实验、热力学分析和表面张力测定，证明它们对细胞的保护作用建立在表面活性基础上。通过改变系统的界面特性阻止细胞在气泡表面的吸附，从而有效地消除气泡对细胞的破损作用。

Here the protective effect and mechanism of serum and Plurc ic F68 on hybridoma cells in suspended cultures were examined and analyzed by.a series of experimental and theoretical studies.It is,found that serum and Pluronic F68 do not Show and protection on cells when the Kolmogorov turbulent eddy sizes of the bulk liquid in bioreactors are similar to or smaller than the cell size. However,if the gas bubbles are the major source to result in cell damage,their potective effect is very significant.Particularly,Pluronic F68,at its concentration of lg/L provides full prevention to cells from damage by bubbles. The results of foam fractionation experiments,thermodynamic analysis and surface tensioin measurements demonstrate that the protective effect of serum and Pluronic F68 on cells is based on their surface activities,in which they eliminate cell damage by changing the interfacial properties of the system to prevent cell-bubble adhesion.