摘要
采用Bradford比色法测定西藏嘉黎、亚东两地区牦牛血清总蛋白含量,通过醋酸纤维薄膜电泳分离其血清蛋白各组成,并以CDS200型计算机控光密度仪进行扫描.测得49头嘉黎牦牛的血清总蛋白含量为6.28±0.34g/100ml血清,其中各组成的百分含量分别是:白蛋白45.59±0.92,α1球蛋白17.06±0.40,α2球蛋白8.97±0.68,β球蛋白10.51±0.50,γ球蛋白17.75±0.70,清球蛋白比值(A/G)为0.84±0.02;36头亚东牦牛的血清总蛋白含量为6.45±0.20g/100ml血清,其中各组成的百分含量分别是:白蛋白46.66±1.10,α1球蛋白17.48±1.02,α2球蛋白8.85±0.66,β球蛋白9.63±0.74,γ球蛋白17.42±0.98,清球蛋白比值(A/G)为0.88±0.04.经t检验分析,两地区牦牛血清蛋白组成中只有β球蛋白含量具有显著差异(P<0.05),其余均无显著性差异(P>0.05).
The contents and components of serum protein in Tibetan Jiali and Yadong yaks were analysed.The contents of serum protein in 49 Jiali and 36 Yadong yaks were 6.28±0.34g/100ml and 6.45±0.20g/ml.The components percent of their serum albumin,α1 globulin,α2 globulin,β blobulin,γ globulin were 45.59±0.92,17.06±0.40,8.97±0.68,10.51±0.5,17.75±0.7 and 46.66±1.10,17.48±1.02,8.95±0.66,9.63±0.74,17.42±0.98. Compared the results of analysis between Jiali and Yadong yaks,only the percent of β globulin was significantly different(P<0.05).
出处
《西南民族学院学报(自然科学版)》
1996年第4期418-420,共3页
Journal of Southwest Nationalities College(Natural Science Edition)