应用RT-PCR方法检测腺病毒介导的nm23-H1基因在A549细胞中的表达

RT-PCR for detection of expression of nm23-H1 gene in A549 cell line mediated by adenovirus

目的:应用RT-PCR方法检测腺病毒介导的nm23-H1基因在A549细胞中的表达。方法:制备nm23-H1重组腺病毒并感染人类肺腺癌A549细胞系,用RT-PCR方法检测A549细胞内nm23-H1基因表达量的变化。结果:RT-PCR结果显示,实验组和对照组的A549细胞nm23-H1/β-actin值分别为0.82和0.51(P<0.05)。结论:重组腺病毒载体Adeno-nm23-H1成功介导了A549细胞内nm23-H1基因表达量的上调,为进一步研究nm23-H1抑制肿瘤转移分子机制及基因治疗奠定了基础。

Objective：To analyze the expression of nmZ3 - H1 gene in A549 ceils transducted by recombinant adenovirus. Methods： A.549 cells were transdueted with Adeno - nm23 - HI. The expression of nm23 - HI gene in this cell was analyzed by reverse transcriptase polymerase chain reaction. Results：RT-PCR result showed the level of nm23 -HI mRNA in A549 cells transdueted with Adeno -nm23 - H 1 was higher, nm23 - H1/13 -actin was 0. 82 in trasdueted cells and 0. 51 in non -transducted cells （ P 〈 0. 05）. Conclusion：Adeno- nm23- H1 successfully mediates the overexpression of nm23- H1 gene in A549 cells.