微囊化Leydig细胞异种移植的实验研究

Experimental studies on xenotransplantation of microencapsulated leydig cells

为探索一种新的补充睾酮的方法,将新生大鼠睾丸 Leydig 细胞分离、培养后制成微囊化 Leydig 细胞,移植于小鼠腹腔。结果显示,微囊对睾酮有良好的通透性;微囊内 Leydig 细胞对人绒毛膜促性腺激素有良好的反应性;异种移植1个月后实验组与实验对照组血中睾酮值分别为1.16±0.28nmol/L 和0.60±0.10nmol/L,2个月后分别为0.62±0.10nmol/L 和0.28±0.05nmol/L,阴性对照组血中测不到睾酮。说明该法制备的微囊能减低 Leydig 细胞异种移植后的免疫排斥反应,为今后临床上开展异种睾丸 Leydig 细胞移植提供了依据。

In order to find a new way to treat testosterone insufficiency,leydig cells of new- born rats were seperated,cultured,microencapsulated and implanted into the intraperitoneal cavi- ty of mice recipients.The results showed that the microencapsule membrance had a good permea- bility to testosterone;leydig ceils in the microencapsule were responsive to the stimulation of hCG;one month after transplantation,the blood testosterone levels in experimental and experi- mental control group were 1.16±0.28nmol/L and 0.60±0.10nmol/L,respectively.In the nega- tive control group,the testosterone level was below the sensitivity of the assay.It is indicated that the microencapsulation can diminish the immune reactionto the leydig cells after transplantation, and that this approach will potentially lead to clinical application.