大鼠同种异体喉移植模型的建立

A rat model of laryngeal ailotransplantation

应用现代显微外科技术,将双侧连接于供体喉的颈总动脉分别与受体一侧的颈总动脉和颈外静脉进行端一侧吻合,共对13只大鼠实施喉异位移植,建立了同种异体喉移植的实验模型。移植动物全部存活,移植术后3天,移植物周围有纤维素性粘连,7天后粘连加重,移植物表面附有灰白色的纤维结缔组织,气管内见分泌物滞留,14天时移植物仍未出现明显的坏死现象。认为以大鼠做喉移植模型是可行的,影响移植术成功的因素除供受体之间主要组织相容性复合物的配合程度外,显微外科操作技术、感染的预防和移植物灌洗技术也至关重要。

A rat model of laryngeal allotransplantation was established by employing an end- to-side anastomosis of donor bilateral common carotid arteries to recipient common carotid artery and external jugular vein separatively.Thirteen allotransplants were performed in 26 SD rats.All recipients survived.Three days after the operation,a visible fibrinous adherence was observed around the laryngeal grafts.By the 7th day,the adherence became intensified.The airway was plugged with mucoid material,and the viable grafts were surrounded by connective tissues.Our results confirmed that the rat model was practicable for laryngeal allograft.Besides the difference of the major histocompatibility between the donor and recipient,the skill of microsurgery,the prevention of infections and the methods of donor organ flushing are all vital to a successful trans- plantation.