臭氧对脱矿釉质再矿化影响的实验研究

Effect of ozone on the remineralization of enamel in vitro

目的通过体外实验了解臭氧对脱矿釉质再矿化的影响。方法将脱矿的牛切牙牙釉质分为空白对照组、氟化物组和臭氧组,进行 pH 循环,扫描电镜观察脱矿釉质表面再矿化后的形态改变,激光扫描共聚焦显微镜观察荧光染料进入脱矿釉质内的量,应用再矿化前后釉质片荧光面积A、总荧光量 TF 和病损平均荧光量 AF 3个参数的差值(ΔA、ΔTF、ΔAF)对再矿化效果进行量化,采用方差分析(SNK 检验)进行统计学处理。结果再矿化后,扫描电镜观察空白对照与臭氧组釉质表面粗糙,多孔隙,不规则;氟化物组釉质表面光滑,孔隙明显变得狭小,规则。3组ΔA分别为(0.31±0.28)×10~4、(1.73±0.63)×10~4、(0.28±0.19)×10~4μm^2,ΔTF 分另0为(0.42±0.34)×10~5、(2.53±0.73)×10~5、(0.48±0.27)×10~5,ΔAF 分别为(3.35±2.55)、(46.51±16.64)、(4.95±3.05)。3组再矿化前的 A、TF 和 AF 的差异无统计学意义(P>0.05)。再矿化后,氟化物组的ΔA、ΔTF 和ΔAF 均显著高于空白对照组和臭氧组(P<0.05);而臭氧组与空白对照组的ΔA、ΔTF 和ΔAF 相比,差异无统计学意义(P>0.05)。结论体外无菌条件下,臭氧对脱矿釉质再矿化无明显的抑制或促进作用。

Objective To investigate the effect of ozone on remineralization of enamel in vitro. Methods Caries-like lesions were prepared in the enamel of extracted cattle incisors with deminerahzing solution. The specimens were treated with deionized water, 1000 mg/kg fluoride, 4.5 mg/L ozone, and pH-cycled. The scanning electron microscope （SEM） was used to observe the surface of enamel specimens. The effect of remineralization was assessed by three lesion parameters （fluorescent area, total and average fluorescence）, which were measured with confocal laser scanning microscope （CLSM）. The data were collected and analyzed by SNK test in ANOVA. Results SEM observation showed that the surface morphology of the deionized water group and ozone group were similar： porous and atactic; but the surface morphology of fluoride group was smooth and the aperture became smaller. The three parameters in the three groups were as follows, respectively. A A were （ 0. 31± 0. 28 ）, （ 1.73 ± 0. 63 ） and （ 0. 28± 0.19） ×10^4 μm^2, ATF were（0.42±0.34）, （2.53±0.73） and （0.48 ±0.27）×10s, ΔAF were （3.35±2.55）,（46.51±16.64） and （4.95 ±3.05）. The fluoride group was significantly greater than deionized water group and ozone group （ P 〈 0. 05 ） ; but there was no significant difference between the deionized water group and ozone group. Conclusions The results of this in vitro study suggest that ozone has no effect on remineralization of enamel blocks.