采用比较基因组杂交方法分析原发性食管癌染色体异常

Analyzing Chromosomal Abnormality in Primary Esophageal Cancer by Comparative Genomic Hybridization

背景与目的:有研究表明原发性食管癌常有染色体的改变,包括染色体基因组异常扩增和缺失。比较基因组杂交技术可以显示这些染色体的异常变化。本实验采用比较基因组杂交技术研究和分析原发性食管癌染色体基因组的变化特点及其与预后的关系。方法:采用比较基因组杂交技术检测16例食管癌组织中染色体的异常改变,并分析染色体异常与预后的关系。研究的病例中7例食管癌术后2年内死亡(对照组),9例术后生存3年以上(生存组)。结果:食管癌患者中多数染色体基因组发生改变,最常见的染色体基因组高扩增频率发生在1q/p,2q/p,3q,5q/p,8q/p,9q/p,11q/p,17和20q/p染色体区段上,在染色体1q/p,4p,9p,18q和xp中常见染色体基因缺失。染色体7q/p和19扩增频率和染色体4q/p和18q缺失频率,生存组与对照组间存在显著性差异。结论:食管癌患者染色体区段基因易发生异常扩增和缺失,生存组与对照组存在明显差异。

BACKGROUND ＆ OBJECTIVE. Some researches have showed that chromosomal abnormalities, including chromosomal regions of gains and losses, usually occur in primary esophageal cancer. The comparative genomic hybridization （CGH） technique can show chromosomal abnormality. This study was to analyze the characteristics of chromosomal abnormity in primary esophageal cancer by CGH, and explore its correlation to prognosis. METHODS, Chromosomal genetic changes in 16 specimens of primary esophageal cancer were detected by CGH. The correlation of chromosomal abnormality to the prognosis was analyzed. Of the 16 patients, 7 died within 2 years after operation （control group）, 9 survived over 3 years after operation （survive group）. RESULTS, Most of the patients had chromosomal genetic changes. The most frequent changes were gains of chromosome arms or regions 1q/p, 2q/p, 3q, 5q/p, 8q/p, 9q/p, 11q/p, 17, and 20q/p, and losses of chromosome arms or regions 1q/p, 4p, 9p, 18q, and xp. The differences in gains of chromosome arms or regions 7q/p and 19 and losses of chromosome arms or regions 4q/p and 18q between control group and survive group were significant. CONCLUSIONS, Abnormal gains and losses of chromosome arms or regions tend to occur in esophageal cancer. The differences between control group and survive group are significant.