摘要
目的:为面神经缺损寻找一种理想的异体神经移植物。方法:取Wistar大鼠胫神经,经Triton X-100和脱氧胆酸钠溶液进行化学去细胞处理。将处理后的神经行组织学染色和免疫组织化学染色;并行异体移植修复面神经缺损,观察其组织相容性。结果:去细胞神经为一中空的神经基质管,其中的细胞和髓鞘成分被有效清除,神经基底膜被保留;异体移植后无明显炎症反应,无排斥和吸收反应,能引导宿主轴突和Schwann细胞增殖。结论:去细胞异体神经移植物具有良好的仿生性和组织相容性,可能用于修复面神经缺损。
Objective： To provide an ideal nerve allograft for repairing facial nerve defects. Methods： The tibial nerves from Wistar rats were treated with solution of TritonX-100 and sodium deoxycholate. The decellularized nerves and fresh nerves were observed by HE staining, Luxol fast blue staining, and immunohistochemical staining for Laminin and S100. Results： The decellularized nerve was an empty basal lamina tube with the integrated basal membrane, where the cells, axons and myelin sheaths were removed effectively. After transplantation of decellularized nerve al- lograft, the outgrowth of the axon and migration of the Schwann cells were observed without excessive inflammation and rejected and absorbed reaction. Conclusion： The decellularized nerve allograft has excellent bionic characteristics and histocompatibility, and may be a substitute for autograft to repair facial nerve defects in human beings.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2007年第1期4-6,共3页
Chinese Journal of Anatomy
关键词
神经移植
同种异体
面神经
nerve transplantation
allograft
facial nerve
