白细胞介素-18基因转染的肺癌细胞与树突状细胞融合体的抗肿瘤作用

Anti-tumor effects induced by fusion of interleukin-18 gene transfected NCI-H460 lung cancer cell line with dendritic cells

目的研究白细胞介素-18（IL-18）基因转染的肺癌细胞与树突状细胞（DC）融合体的抗肿瘤作用。方法（1）从人外周血单核细胞诱导培养DC，与IL-18基因转染的NCI-H460肺癌细胞融合，经免疫磁珠筛选。（2）设转染组（GT组）、空载体转染组（PT组）、未转染组（NT组）和对照组（BC组），分别以IL-18转染的融合细胞、peDNA3．1^＋转染的融合细胞、未转染的融合细胞活化的T细胞为效应细胞，BC组不含效应细胞，用乳酸脱氢酶（LDH）法测定效应细胞对NCI—H460细胞的杀伤作用。（3）荷瘤裸鼠皮下注射上述3种效应细胞，以BC组为空白对照，比较4组裸鼠肿瘤体积和重量。结果3组效应细胞在体外对NCI-H460细胞的杀伤率分别为53．1％，30．1％和31．5％；3组裸鼠肿瘤体积及肿瘤重量明显低于BC组，以GT组最低。结论IL-18基因转染的融合细胞可有效诱导抗肿瘤免疫。

Objective To explore the anti-tumor effects induced by fusion of interleukin （IL）-18 gene transfected lung cancer cell line NCI-H460 cells with dendritic cells （DC）. Methods （ 1 ） DC were induced from human monocytes and fused with IL-18 transfected NCI-H460 ceils. Fusion was selected using MACS microbeads. （2）Four groups （group GT, group PT, group NT and group BC ） were set up. T cells activated by IL-18 gene transfected fusion or pcDNA3.1^＋ vector transfected fusion and non-transfected fusion were taken as effetor cells, No effector cells was in group BC, Lactic dehydrogenase （LDH） method was used to evaluate the antitumor effect in vitro, （ 3 ） Tumor-bearing nude mice were inoculated with effector cells mentioned above. The tumor size and weight in the 4 groups were compared, Results The killing rate in vitro of 3 groups were 53, 14% ,30.10% and 31,49% , respectively. The tumor size and weight in the 3 groups were lower than group BC, among which group GT was the lowest. Conclusion Fusion of IL-18 gene transfected NCI-H460 lung cancer cells with dendritic cells can effectively induce anti-tumor immunity in the host.