5,10-亚甲基四氢叶酸还原酶基因C677T突变与再发脑梗死的关系

The relationship between C677T mutation of 5,10-methylenetetrahydrofolate reductase and recurrent cerebral infarction

目的探讨5,10-亚甲基四氢叶酸还原酶(MTHFR)基因C677T突变与再发脑梗死的关系。方法收集脑梗死患者及对照组的血液标本,采用聚合酶链式反应-限制性片段长度多态性分析法分析其MTHFR基因C677T突变。结果初发脑梗死组MTHFR基因C677T突变率为68.0%,再发脑梗死组突变率为69.5%,均高于对照组46.3%,差异均有显著性(P<0.05);初发脑梗死组与再发脑梗死组MTHFR基因C677T突变率没有显著性差异(P>0.05)。结论MTHFR基因C677T突变可能与脑梗死的发病有关,但与脑梗死发病的次数无关。

Objective To explore the relationship between C677T mutantion of 5,10-methylenetetrahydrofolate reductase （MTHFR） and recurrent cerebral infarction. Methods Blood samples were collected from the cerebral infarction patients and controls. The CA77T mutation of MTHFR was determined by the method of polymerase chain reaction and restriction fragment length polymorphism. Results The CA77T mutant frequencies of MTHFR in group of both primary and recurrent cerebral infarction were 68. 0 % and 69. 5 % respectively which were higher （P〈0. 05） than that in controls 33. 8%. The C677T mutant frequencies of MTHFR in group of both primary and recurrent cerebral infarction had no significant difference（P〉0. 05）. Conclusions The C677T mutant frequency of MTHFR might be correlated only with cerebral infarction but not with the frequency of onset of cerebral infarction.