灵芝孢子油与深海鱼油联合应用对小鼠学习记忆及海马神经元表达NOS的影响

Effects of combination of the ganoderma spore oil and deep sea fish oil on learning and memory ability and NOS expression of hippocampal neuron in mouse

目的探讨灵芝孢子油与深海鱼油联合应用对小鼠学习记忆及海马神经元表达NOS的影响。方法将受孕小鼠随机分为4组,自受孕第1天起分别胃饲生理盐水、灵芝孢子油、深海鱼油和灵芝孢子油加深海鱼油。在母鼠分娩21d后改为胃饲其幼鼠,然后将出生后45d的幼鼠处死。处死前进行Morris水迷宫行为学测试,处死后应用酶组织化学法检测海马神经元NOS的表达。结果在Morris水迷宫检测中,灵芝孢子油组、深海鱼油组和灵芝孢子油加深海鱼油组小鼠的逃逸潜伏期明显缩短。灵芝孢子油组和灵芝孢子油加深海鱼油组的小鼠平台象限游泳距离有增加。灵芝孢子油加深海鱼油组的小鼠大脑海马NOS阳性神经元与其它组的小鼠比较有显著性增加。结论灵芝孢子油和深海鱼油联合应用能够促进小鼠学习记忆能力及其大脑海马神经元表达NOS。

Objective To explore the effects of combination of the ganoderma spore oil and deep sea fish oil on learning and memory ability and NOS expression of hippocampal neurons in mouse. Methods Pregnant mice were divided into four groups： control group, ganoderma spore oil group, deep sea fish oil group and combination of ganoderma spore oil and deep sea fish oil group. Saline, ganoderma spore oil, deep sea fish oil and combination of ganoderma spore oil and deep sea fish oil was administered to the pregnant mice respectively from the 1st day of encyesis （Eld）, and then to the young mice from the 21st day after parturition （P21d）. The young mice from P45d were sacrificed after the test of Morris water maze finished. NOS expression of hippocampal neurons was detected with enzymohistochemistry. Results The escape latency obviously shortened on the test of Morris water maze in the ganoderma spore oil group, deep sea fish oil group, combination of ganederma spore oil and deep sea fish oil group of mice. The swimming distance of platform quadrant increased in the ganoderma spore oil group, combination of ganederma spore oil and deep sea fish oil group of mice. Compare with other groups of mice, NOS positive neurons of cerebral hippocampus enhanced significantly in the combination of ganederma spore oil and deep sea fish oil group of mice. Conclusion The combination of ganederma spore oil and deep sea fish oil may promote the learning and memory ability and NOS expression of hippocampal neurons in mouse.