摘要
本文旨在探讨守宫木的组织培养技术和方法。实验采用守宫木带腋芽的茎段为材料。实验结果表明:启动和增殖培养以MS+BA 1.5mg/L+NAA 0.1 mg/L+琼脂6g/L+蔗糖30g/L的效果最好;生根培养以MS+NAA 0.1 mg/L+琼脂6g/L+蔗糖20g/ L的效果最好:在腐质土介质中移栽成活率可达95%。结果说明守宫木带腋芽的茎段离体繁殖,增殖系数大,适合于种苗快速繁殖。
This paper focuses on the methodology of tissue culture of Sauropus androgynus (L.) Merr. The stem with auxiliary bud of Sauropus androgynus (L) Merr. was employed as material. The results showed that the best medium for starting culture and multiplication contained MS , BA 1.5mg/L , NAA 0.1 mg/L, agar g/L and sugar30g/L, and the best medium for roofing contained MS, NAA 0. 1 mg/L agar g/L and sugar 20g/L. In humus, the survival rate of transplant was up to 95 %. The results also showed that the stem with auxiliary bud was good for rapid propagation of Sauropus androgynus ( L. ) Merr
关键词
守宫木
组织培养
技术
Sauropus androgynus (L.) Merr
tissue culture
technology