山羊FSHR基因第10外显子序列的克隆与比较研究

Cloning and Sequence Analysis of Exon10 of FSHR Gene in Goat

根据牛FSHR基因序列设计引物,以波尔山羊和莱芜黑山羊基因组为模板,应用PCR技术克隆测定了FSHR基因第10外显子序列,并与牛该区段序列进行了比较研究。结果表明,PCR扩增获得的片断为1643bp,三畜种FSHR基因第10外显子长均为1233bp。莱芜黑山羊和波尔山羊的第10外显子序列与牛该序列相比,同源性分别为97.49%和97.41%,分别在31个和32个位点发生碱基突变。均引起10处氨基酸密码改变。波尔山羊该序列与莱芜黑山羊的比,同源性为99.60%,且3个位点有碱基突变,其中+1184和+1365位点突变引起氨基酸密码改变。

The exonl0 of FSHR was amplified by PCR using genomic DNA of boer goats and Laiwu black goats as the template and designing primers according to the sequence of the cow receptor gene. The sequence was determined and analyzed according to cloning ,and the sequence was compared with that of cow FSHR gene. The results indicated that sequence length of FSHR gene was the same and was 1233bp among the three species . The exon10 sequence of the FSHR gene of the LaiWu black goat and boer goat compared with that of the cow, the homology were 97.49% and 97.41% respectively ,and each has 31 and 32 mutated bases and caused 10 amino acid code changes. While the exon10 sequence of FSHR of boer goat compared with LaiWu black goat,the homology was 99.60%,there were 3 mutations between them, that caused amino acid code changed respectively at ＋1184bp and ＋ 1365bp of the exon10 sequence of the FSHR.