摘要
采用牛病毒性腹泻病毒(BVDV)免疫产蛋母鸡,然后用水稀释法成功地从卵黄中分离出抗BVDV免疫球蛋白(抗-BVDV IgY)。建立起测定鸡蛋中特异性抗BVDV活性的间接ELISA方法,并采用此方法检测每次免疫后第10天的抗体滴度。结果表明,蛋鸡在首次免疫后第10天,卵黄中可以检测到抗-BVDV IgY;5次免疫后,抗体效价达到1:6400,半年后仍能维持在该水平。
Layer hens were immunized with Bovine viral diarrhea virus （BVDV）, the egg yolk was successfully treated with water diiution method. The IgY titer, was 10days after every immunization, was detected by indirect ELISA. The results showed that we can detect anti-BVDV IgY in the first 11days. After being immunized with 5 doses of the conjugate, the titer was 1-6400 and kept the conditions after 6 months.
出处
《家畜生态学报》
2007年第1期75-78,共4页
Journal of Domestic Animal Ecology
基金
科技部农业成果转化资金项目(03EFN216510266)资助
