RAPD技术鉴定商品药材百合

Identification of Commercial Crude Drug Bulbus Lilii with RAPD Technique

目的鉴别商品药材百合及其混淆品,探讨商品药材鉴别的新方法。方法采用RAPD技术对商品药材百合进行了多态性分析,并构建聚类树型图。结果RAPD技术能从分子水平上检测种内差异,可以解决不同产地的商品药材百合与混淆品轮叶百合不易区分的难题。结论RAPD技术完全可以作为商品药材的一种较好的鉴别方法。

Objective To identify the commercial medicinal materials Bulbus Lilii and the confused materials, and investigate a new identifying method for the medicinal materials. Methods Polymorphic analysis was used for the commercial crude drug Bulbus Lilii with RAPD technique and a tree diagram was constructed. Result The RAPD technique could detect the differences between the different materials within the molecule elevel and solve the difficult problem of the commercial crude drug Bulbus Lilii confused with the other ones. Conclusion The RAPD technique can be taken as a effective identification for commercial crude drug Bulbus Lilii.