Notch2、Notch4在新生早产大鼠高氧肺损伤中的表达

Expression of Notch2,Notch4 in Hyperoxia Induced Lung Injury in Premature Rats

目的探讨Notch2、Notch4在早产大鼠高氧肺损伤发生发展中的作用。方法将生后1d内早产SD大鼠随机分为高体积分数氧暴露组和空气组。于生后1、7、14、21d留取肺组织标本,免疫组织化学法检测Notch2、Notch4的表达,RT-PCR检测Notch2、Notch4 mRNA的表达。结果在肺发育不同时期,Notch2、Notch4表达具有各自不同的规律,吸氧体积分数为85%氧暴露不同程度改变了它们的表达。结论在吸氧体积分数为85%的氧气长期暴露,Notch2、Notch4异常表达,可能是早产大鼠肺损伤的发生机制之一。

Objective To explore the roles of Notch2, Notch4 in hyperoxia induced lung injury in premature rats. Methods At the postnatal 1 day Sprague - Dawley premature rats were randomly assigned to about 85% hyperoxia group and air group. At the 1,7,14,21 days after exposed ,8 rats of each group were used to evaluate expressions of Notch2, Notch4 in lungs by immunohistochemistry and the level of Nothch2, Notch4 mRNA by reverse transcription polymerase chain reaction（ RT - PCR）. Results Expressions of Notch2, Notch4 had their rules in rats＇ different stages of development;85% oxygen exposed would change their tracks. Conclusion Prolonged 85% oxygen exposure result in abnormal expressions of Notch2 and Notch4 , which is likely to lead to the pathogenesis of hyperoxic lung injure in premature rats.