不同CO浓度对ALI大鼠肺炎性变化的影响

Effect of different concentrations of carbon monoxide on inflammation reaction of rats with acute lung injury

目的探讨减轻ALI(急性肺损伤)大鼠肺炎性改变的最佳一氧化碳浓度。方法随机将30只条件相当的大鼠分为五组:正常对照组,LPS(大肠杆菌内毒素)组,LPS+3%CO组,LPS+8%CO组,LPS+15%CO组。24h测动脉血和支气管肺泡灌洗液(BALF)中细胞因子IL-8、TNF含量。并取五组大鼠肺组织,做病理切片进行比较。结果LPS组,在静脉注射LPS后24h其动脉血和BALF中细胞因子IL-8、TNF含量明显增加,显著高于正常对照组;LPS+3%CO组细胞因子IL-8、TNF含量虽有增高,但与正常对照组之间无明显差异;LPS+8%CO组,LPS+15%CO组与LPS组无明显差异;病理切片的比较亦提示LPS+3%CO组其炎性改变最轻。结论给大鼠静脉注射LPS造成ALI的同时给予低浓度(3%)CO气体吸入,可降低细胞因子水平从而减轻急性肺损伤的炎性反应。

Objective To explore the best concentration of carbon monoxide （O3） to decrease inflammation reaction in the lipopolysaeeharide （LPS）-indueed acute lung injury （ALI）. Methods The rats were divided into five groups： control group,LPS group, LPS ＋ 3 % 133 group, LPS ＋ 8 % 133 group, and LPS ＋ 15 % （33 group. The concentration of eytokine, interleukin - 8 （ IL-8 ） and tumor necrosis factor （TNF）, in carotid artery and bronehoalvcolar lavage fluid （BALF）from male SD rats were determined at hour 24 after injection with LPS via caudal vein. Then pathological sections of the lungs were compared. Results The eytokine of carotid artery and BALF was significantly higher in the rats at hour 24 after LPS injection than that in control group （P〈 0.01 ）. The level of eytokine in LPS ＋ 3%（39 group was slightly higher than that in control group, but there was no significant difference between two groups （P〉0.05）. But the levels of eytokine in LPS＋ 8%（39 group, LPS＋ 15%（39 group and LPS group were not obviously different. The pathological sections also showed that the inflammation reaction in LPS ＋ 3 % （3D group was the lightest. Conclusion It clearly suggests that low concentration（3% ）of （33 in ALI rats can play an important role and it can decrease inflammation reaction of ALI.