应用复合PCR检测水稻种子的转基因成分

Rapid Detection of the Transgenic Rice Seeds by Multiplex Polymerase Chain Reaction(MPCR)

根据转基因水稻中常用的花椰菜花叶病毒(CaMV)35 S启动子、胭脂碱合成酶(NoS)终止子、β-葡萄糖苷酸酶基因(GUS)、潮霉素磷酸转移酶基因(Hpt)、新霉素磷酸转移酶基因(NptII)和B t毒蛋白基因(B t Cry 1 Ab)的序列,设计合成6对不同的引物,用简单PCR方法检测了水稻种子中的转基因成分,同时建立应用一次PCR反应同时扩增检测两种或多种外源基因的复合PCR方法。通过对水稻样品进行PCR扩增,分析、比较复合PCR的检测结果与简单PCR的扩增结果,发现两者结果完全一致。表明复合PCR方法不但可以提高检测效率、降低检测成本,还可以有效防止假阳性,是一种值得推广的方法。

According to the specific sequences of 6 transgenes, CaMV 35 S, NoS, GUS, Hpt, NptII and Bt Cry 1 Ab,6 pairs primers were designed. The transgenic components of rice varieties were testified through single PCR reaction. We describe a rapid method capable of detection genetically modified rice with multiplex polymerase chain reaction （ MPCR）, at the same time we can detect several target genes with this method. The MPCR is a rapid, efficient and inexpensive method.