抗体差异度法推测血吸虫病患者感染时间的探讨

Investigation of infection time of schistosomiasis japonica with antibody difference ratio method

目的探讨抗体差异度法推测血吸虫病患者感染时间的可行性。方法检测小鼠和急性血吸虫病患者血清中的IgM、IgG抗体水平，并分别计算抗体差异度值，推测急性血吸虫病患者的感染时间；通过循环抗原检测和免疫印迹反应验证急性血吸虫病患者的感染情况，并与现场流行病学调查资料相比对。结果经抗体差异度值分组的急性血吸虫病患者的感染时间明显不同，差异度值大于0．61的患者感染时间约为4-5周，而小于0．61的患者感染时间在7-8周以上。结论抗体差异度法在推测急性日本血吸虫病患者的感染时间方面具有可行性。

Objective To investigate the feasibility of antibody difference ratio method for prediction of infection time of schistosomiasis japonica. Methods The antibody difference ratio was calculated based on the values of specific IgM and IgG antibodies in sera from infected mice and acute schistosomiasis patients, The infection time of acute schistosomiasis patients was assessed with their epidemiology data, and circulating antigens in sera and fractionated antigens of soluble eggs antigen （SEA） were analyzed as indirect evidences. Results All acute schistosomiasis patients could be derided into two groups according to the difference ratio of IgM-ELISA over IgG-ELISA. The average infection time of the first group with difference ratio of more than 0.61 was about four to five weeks, while that of the second group with difference ratio of less than 0.61 was more than seven to eight weeks, Conclusion It was feasible to predict the infection time of acute schistosomiasis patients with antibody difference ratio method.