云芝多糖对实验性动脉粥样硬化家兔血浆氧化型低密度脂蛋白含量的影响

The effect of Polysaccharide krestin on plasma oxidized low density lipoprotein levels of experimental atherosclerotic rabbits

用本室制备的两株单克隆抗体（ＨＯＬ２和ＨＯＬ５）比较了经与未经云芝多糖治疗的实验性动脉粥样硬化家兔血浆氧化型低密度脂蛋白的含量。发现血浆氧化型低密度脂蛋白以低、中修饰度低密度脂蛋白为主；血浆氧化型低密度脂蛋白水平与主动脉粥样硬化斑块面积成正相关；云芝多糖能降低血浆氧化型低密度脂蛋白水平。提示利用单克隆抗体检测血浆氧化型低密度脂蛋白含量可初步预测动脉粥样硬化严重程度；云芝多糖降低血浆氧化型低密度脂蛋白水平可能是其减轻动脉粥样硬化斑块面积的机理之一。

Aim To observe the changes of plasma oxidized low density lipoprotein (OLDL)levels of experimental atherosclerotic rabbits with or without polysaccharide krestin(PSK)treatment.Methods All rabbits were fed standard chow supplemented with 1% cholesterol and 4% lard for 12weeks,then injected introperitoneally with 2.5% PSK (or saline)3.5 ml.Plasma levels of OLDL were measured by HOL2 and HOL5 monoclonal antibodies using ELISA method at 0,4,8 and 12 week after treatment.The atherosclerotic lesion areas were determined by computer.Results We found that there is positive correlation between plasma OLDL levels and the area of atherosclerotic plaque of atherosclerotic rabbits both in PSK treated group and in control group,detected either by HOL2 or by HOL5.Moreover,striking decrease of plasma leves after PSK treatment was found and there was signif cant decrease between two groups at 8 and 12 week(P<0.01 )Conclusions The results suggested that OLDL level in plasma detected by monoclonal antibodies is a good index to reflect the degree of atherosclerotic lesion.Furthermore,by using monoclonal antibodies,we present direct evidence that PSK can decrease plasma OLDL levels,espesially highly oxidized LDL, and find this effect is in accordance with the decrease of atherosclerotic plaque area.