摘要
利用由纯化的人血浆载脂蛋白E制备获得的小鼠抗人载脂蛋白E单克隆抗体和兔抗人载脂蛋白E多克隆抗体,建立了测定人血清载脂蛋白E浓度的抗体双夹心酶联免疫吸附测定方法。该方法具有特异性强、灵敏度高、操作简便和重复性好等优点,可广泛地应用于基础研究和临床检验。
A sandwich enzyme linked immunosor bent assay(ELISA)for quantitation of human serum apolipoprotein E was developed with rabbit polyclonal antibodies and horseradish peroxidase labelled mouse monoclonal antibodies against human apolipoprotein E.The estabolished method has been evaluated to have the following advandages:high specificity and sensitivity,easy operation and good reproducibility with 5.17% intraassay CV and 8.62% interassay CV.The developed method tan been widely used in basical med ical reseach and clinical laboratory.
出处
《中国动脉硬化杂志》
CAS
CSCD
1996年第3期217-220,共4页
Chinese Journal of Arteriosclerosis
基金
天津市二十一世纪青年科学基金
卫生部科学基金