人血清载脂蛋白E酶联免疫吸附测定方法的建立

Development of Enzyme Linked Immunosorbent Assay for Human Serum Apolipoprotein E

利用由纯化的人血浆载脂蛋白Ｅ制备获得的小鼠抗人载脂蛋白Ｅ单克隆抗体和兔抗人载脂蛋白Ｅ多克隆抗体，建立了测定人血清载脂蛋白Ｅ浓度的抗体双夹心酶联免疫吸附测定方法。该方法具有特异性强、灵敏度高、操作简便和重复性好等优点，可广泛地应用于基础研究和临床检验。

A sandwich enzyme linked immunosor bent assay(ELISA)for quantitation of human serum apolipoprotein E was developed with rabbit polyclonal antibodies and horseradish peroxidase labelled mouse monoclonal antibodies against human apolipoprotein E.The estabolished method has been evaluated to have the following advandages:high specificity and sensitivity,easy operation and good reproducibility with 5.17% intraassay CV and 8.62% interassay CV.The developed method tan been widely used in basical med ical reseach and clinical laboratory.