降钙素基因相关肽参与电针抗急性心肌缺血损伤的实验研究

CGRP May Take Part in the Effect of Electroacupuncture in Resisting Acute Myocardial Ischemic Injury

目的:探讨电针刺激抗心肌缺血的机制。方法:采用异丙肾上腺素(Isoprenolonhydrochloride,ISO)腹腔注射建立大鼠急性心肌缺血实验模型。24只雄性Wistar大鼠随机分为3组,每组8只。①电针治疗组:将大鼠麻醉后,注射2%ISO,按100mg/kg(即5mL/kg),i.p.,同时进行电针治疗。选取左侧“心俞”接正极和“厥阴俞”接负极。电针参数:输出波形为正负向交替脉冲波,脉冲宽度为300μs,频率2-20Hz,疏密波,输出电压为3.5-5V,以针柄轻度抖动为度,每次电针30min,共2次,间隔12h。②模型组:同电针治疗组,但不进行电针治疗。③对照组:将大鼠麻醉后,注射生理盐水,38℃,按5mL/kg体重i.p.。比色法测定血清肌酸激酶(creatinekinase,CK)值;放射免疫法测定血浆、心脏组织、脊髓(T1-T6)组织内降钙素基因相关肽(CGRP)含量。结果:电针治疗能够降低造模引起的血清CK水平升高;急性心肌缺血损伤可导致血浆CGRP含量降低,但心肌组织和脊髓中CGRP水平无明显变化;电针后,大鼠血浆中CGRP含量升高(P<0.01),心肌组织内CGRP含量仍无明显变化,但脊髓中CGRP含量有所升高(P<0.05)。对血浆、心脏组织及脊髓组织内的CGRP进行相关分析,未发现三者的明显相关性。结论:电针刺激具有一定的抗心肌缺血损伤作用,CGRP可能是参与电针抗心肌缺血的重要因子。

Objective： To probe into the relationship between ＂Xinshu＂ （BL 15） - ＂Jueyinshu＂ （BL 14） of the Bladder Channel and the heart and the underlying mechanism of electroacupuncture （EA） in resisting acute myocardial ischemia （AMI） in rats. Methods： A total of 24 mate Wistar rats were randomly divided into control, model and EA groups with 8 cases in each group. AMI model was duplicated by intraperitoneal injection of 2 % isoproterenol （ISO, 100 mg/kg）. EA （pulse duration 300 us, frequency 2-20 Hz, output voltage 3.5-5 V） was applied to BL14 and BL15 on the left side for 30 min, twice in a day （with an interval of 12 h）. After decapitation, blood samples, myocardial tissue and spinal cord （T1-T6） were collected respectively for detecting serum creatine kinase （OK, with chromatometry） and calcitonin gene-related peptide （CGRP） contents in the plasma, myocardium and the spinal cord （with radioimmunoassay）. Results： In comparison with control group, serum OK of both model group and EA group increased significantly （P〈0. 01 ）, and plasma CGRP content of model group lowered lightly; while compared with model group, OK content of EA group decreased obviously （ P〈0. 05） and plasma CGRP of EA group increased evidently （P〈0.05）. No significant differences were found among 3 groups in myocardial CGRP contents and between control group and model group in spinal CGRP contents （P〉0.05）. Conclusion： EA of ＂Xinshu＂ （BL 15）-＂Jueyinshu＂ （BL 14） may suppress isoproterenol-injection induced myocardial injury and plasma CGRP possibly takes part in the protective effect of EA in resisting myocardial ischemia injury.