胃癌胎盘型GST－π表达的意义

Placental GST_π expression in human gastric carcinoma

目的研究胃癌组织新标志酶ＧＳＴ＿π的表达和它的临床意义。方法胃癌组织６２例用抗ＧＳＴ＿π抗体，按ＡＢＣ法进行免疫组织化学检测癌组织中ＧＳＴ＿π活性，并与正常胃粘膜，异型增生及肠上皮化生进行比较。结果胃癌组织６２例中ＧＳＴ＿π阳性率８７１％；２７例正常胃粘膜ＧＳＴ＿π阳性率只１１１％（Ｐ＜０００１）；异型增生和肠上皮化生各２３例，其ＧＳＴ＿π阳性率分别为８２６％和８６９％，与正常胃粘膜比较Ｐ＜０００１。结论ＧＳＴ＿π在胃癌组织中的胎儿型表达为研究消化道肿瘤早期诊断和发病机理提供了新的酶学标志。具有中等度异型增生和不全结肠型上皮化生的慢性萎缩性胃炎阳性率也较高，可列为胃切除手术的相对适应证。

AIMS To study the expression of oncofetal glutathione S_tranferase(GST_π) as a new tumor marker in gastric carcinoma and its clinical significance. METHODS GST_π was detected in 62 human gastric carcinoma by immunohistochemical technique(IHCT) with ABC method using anti_GST_π antibody, compared with normal gastric membrane, atypical hyperplasa and intestinal metaplasia. RESULTS GST_π positive staining was 87 1% in 62 huamn gastri carcinoma and 11 1% in 27 normal gastric membrance (P<0 01); 82 6% and 86 9% in 23 atypical hyperplasia and intestinal metaplasia. Compared with normal gastric membrane the differance was very significant ( P <0 01). CONCLUSIONS The expression of oncofetal GST_π is a new tumor marker for human gastric carcinoma in early diagnosis. Chronic atrophic gastritic with moderatly aplasia and intestinal metaplasia of colon type with GST_π positive staining may be a relative indication of gastric operation if the patient's symptom is more severe.