喉鳞状细胞癌组织中CTGF和OPN的表达及其临床意义

Expression of connective tissue growth factor(CTGF),osteopontin(OPN)and clinical significances in the largngeal squamous cell carcinoma tissues

目的:研究人喉鳞状细胞癌及其癌旁组织中CTGF和OPN表达水平并探讨两者的相互关系及其临床病理意义。方法:41例喉鳞状细胞癌和20例癌旁组织手术切除标本常规制作石蜡包埋切片,CTGF和OPN染色方法为SP免疫组织化学法。结果:41例鳞状细胞癌CTGF和OPN表达阳性率均为25例(61.0%),其评分值分别为2.41±1.60和3.10±1.63,20例癌旁组织CTGF阳性18例(90%)和OPN阳性3例(15.0%),其评分值分别为4.24±1.42和1.12±0.84,癌组织CGTF阳性率及评分明显低于癌旁组织(χ2=5.44,P<0.05;t=4.36,P<0.01),但OPN则相反(χ2=11.44,P<0.01,t=5.08,P<0.01);颈淋巴结未转移和临床分型T1病例CTGF表达阳性率及其评分明显高于颈淋巴结转移和临床分型T3病例(P<0.01);颈淋巴结转移、临床分型T1及组织学分级Ⅰ级病例OPN表达阳性率及其评分明显低于颈淋巴结转移、临床分型T3及组织学分级Ⅲ级病例(P<0.01);CTGF表达评分与OPN表达评分呈密切负相关(r=-0.56,P<0.01)。结论:CTGF和OPN可能是反映喉鳞状细胞癌进展、生物学行为、转移发生及预后的重要生物学标记物。

Objective：To study the expressions of CTGF and OPN and detect their clinical value and correlation in the largngeal squamous cell carcinoma tissues and paracancerous tissues. Method：SP immunohistochemical method was used for the assayes of CTGF and OPN on the routinely paraffin-embedded sections of surgical operated specimens of 41 cases with largngeal squamous cell carcinoma and 20 ones with paracancerous tissues. Result： the positive rate of CTGF and the score were significantly lower in cancer tissues than those in paracancerous tissues （61.0 % vs 90. 0 %, P 〈 0.05 % 2.41± 1.60 vs 4.24 ± 1.42, P 〈 0.01 ）, but those of OPN wereopposite （61.0% vs 15.0%, P〈0.01% 3.10±1.63 vs 1.12±0.84, P〈0.01）. The positive rates and scores of CTGF were significantly higher in the cases without-metastasis of lymphnode and clinical stage T1 than those in the ones with-metastasis of lymphnode and clinical stage T3 （P〈0.01） . The positive rates and scores of OPN were significantly lower in the cases without-metastasis of lymphnode , clinical stage T1 and histological grade Ⅰ those that in the ones with-metastasis of lymphnode, Clinical stage T3 and histological grade Ⅲ （P〈0. 01）. The closely negative correlation was found between the score of CTGF and that of OPN. Conclusion：The expression of CTGF and / or OPN migh be important biological markers in reflecting the progression, biological behaviors, metastatic potential and prognosis of the laryngeal squamous cell carcinoma.