钙磷对体外培养SD大鼠破骨细胞生成和活化的影响

Effects of calcium and phosphorus on the formation and activation of SD rat's osteoclasts cultured in vitro

通过成骨细胞与脾细胞共培养,在体外诱导脾细胞转化为破骨细胞,转化过程中分别加入3、5mmol/L的钙或磷及不同比例的钙、磷,设不添加钙、磷因子对照。经形态学观察、抗酒石酸酸性磷酸酶(TRAP)染色计数、扫描电镜观察象牙片吸收陷窝研究钙、磷因子对大鼠破骨细胞生成和活化的影响。结果表明,培养液中钙、磷浓度分别为35、mmol/L时对破骨细胞的生成及活化均有显著抑制作用(P<0.05),5mmol/L Ca对破骨细胞生成活化的抑制与对照组差异极显著(P<0.01)。CCa∶CP=2∶1组对破骨细胞生成和活化的抑制作用最强。证实钙、磷通过抑制破骨细胞的生成和活化抑制破骨细胞的骨吸收活性。

Osteoclasts（OC） were obtained by a co culture system with SD rat spleen cells and osteoblasts. In the conversion process ,different strengths of calcium, phosphorus and different concentration ratios of calcium to phosphorus were added to the co-culture system, respectively. The formation and activation of OC was detected by morphologic observation, histochemical staining for tartrate-resistant acid phosphatase （TRAP） and detection of lacunar resorption through scanning electron microscopy, respectively. The formation and activation of OC was inhibited by high concentrations of calcium and phosphorus （P〈 0. 05）. , and the difference between the high concentration group and the control group was very significant. Moreover,the formation and activation was inhibited mostly by the ratio of calcium to phosphorus （2 ：1）. The results demonstrated that calcium and phosphorus inhibit bone absorbing activity by inhibiting the formation and activation of OC.